This renewal application seeks to investigate how vitamin A regulates mucous cell differentiation in airway epithelium. Two stages of mucous cell differentiation are proposed. In the first stage, cells are unable to differentiate into mucous cells in response to vitamin A. However, they can be transformed into mucin-producing cells by microinjection of RNA from differentiated mucous cells. This first stage is therefore termed the """"""""competent"""""""" stage of mucous cell differentiation, and vitamin A can cause immortalized airway epithelial cell lines to become competent. In the second """"""""progressive"""""""" stage, vitamin A induces mucous cell differentiation. Dr. Wu has identified one to three genes from progressive stage cells which cause stage 1 cells to express mucins. This proposal seeks to clone and to characterize these genes, to define the level of regulation by vitamin A (i.e., transcription or translation), to elucidate the expression of these genes in airway epithelium, and finally to define the mechanism by which these genes induce mucous cell differentiation.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37HL035635-12
Application #
6017227
Study Section
Special Emphasis Panel (ZRG2-RAP (01))
Project Start
1987-04-01
Project End
2000-05-31
Budget Start
1999-06-01
Budget End
2000-05-31
Support Year
12
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California Davis
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
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