Abstract

The long term goal of the research in this proposal is to understand the neural mechanisms controlling the expression of arousal. The research will use combined behavioral, biochemical, and electrophysiological approaches to investigate the role of peptide transmitters and co-transmitters in the expression of arousal states. lt is hypothesized that intrinsic co-transmitter modulators in buccal motoneurons function to stabilize motor contractions under varying conditions. Extrinsic modulatory systems may override this autoregulation to produce plasticity in the system, which may be needed during different behavioral states or during different classes of behavior executed by the buccal mass. The proposed projects include: 1) Purification and sequencing of bioactive peptides In the ARC muscle. 2) Study of the characteristics of the release of peptides from motoneurons. 3) Localization and functional characterization of peptidergic neurons innervating the ARC muscle. 4) Analysis of a model of the functional organization of the motor control of the buccal mass.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37MH036730-13
Application #
2244489
Study Section
Special Emphasis Panel (NSS)
Project Start
1990-05-01
Project End
1998-04-30
Budget Start
1994-05-01
Budget End
1995-04-30
Support Year
13
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Mount Sinai School of Medicine
Department
Physiology
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029

  Publications

Romanova, Elena V; McKay, Natasha; Weiss, Klaudiusz R et al. (2007) Autonomic control network active in Aplysia during locomotion includes neurons that express splice variants of R15-neuropeptides. J Neurophysiol 97:481-91
Wu, Jin-sheng; Due, Michael R; Sasaki, Kosei et al. (2007) State dependence of spike timing and neuronal function in a motor pattern generating network. J Neurosci 27:10818-31
Proekt, Alex; Jing, Jian; Weiss, Klaudiusz R (2007) Multiple contributions of an input-representing neuron to the dynamics of the aplysia feeding network. J Neurophysiol 97:3046-56
Koh, Hae-Young; Weiss, Klaudiusz R (2007) Activity-dependent peptidergic modulation of the plateau-generating neuron B64 in the feeding network of Aplysia. J Neurophysiol 97:1862-7
Orekhova, Irina V; Alexeeva, Vera; Church, Paul J et al. (2003) Multiple presynaptic and postsynaptic sites of inhibitory modulation by myomodulin at ARC neuromuscular junctions of Aplysia. J Neurophysiol 89:1488-502
McDearmid, Jonathan R; Brezina, Vladimir; Weiss, Klaudiusz R (2002) AMRP peptides modulate a novel K(+) current in pleural sensory neurons of Aplysia. J Neurophysiol 88:323-32
Orekhova, I V; Jing, J; Brezina, V et al. (2001) Sonometric measurements of motor-neuron-evoked movements of an internal feeding structure (the radula) in Aplysia. J Neurophysiol 86:1057-61
Karhunen, T; Vilim, F S; Alexeeva, V et al. (2001) Targeting of peptidergic vesicles in cotransmitting terminals. J Neurosci 21:RC127
Xin, Y; Weiss, K R; Kupfermann, I (2000) Multifunctional neuron CC6 in Aplysia exerts actions opposite to those of multifunctional neuron CC5. J Neurophysiol 83:2473-81
Vilim, F S; Cropper, E C; Price, D A et al. (2000) Peptide cotransmitter release from motorneuron B16 in aplysia californica: costorage, corelease, and functional implications. J Neurosci 20:2036-42

Showing the most recent 10 out of 58 publications