A major obstacle to the identification and analysis of genes involved in cell transformation and tumor progression is the need for better methods for capture and stabilization of transcripts in tumor cells. Presently, cDNA populations synthesized from tumor cells are likely to incomplete, in terms of both the length of the product, and the representation of rare sequences. Therefore, cDNA probes and libraries, as well as the amplified products produced from biased and incomplete RNA populations, are less likely to accurately reflect the gene expression profile of the tumor.

Proposed Commercial Applications

When developed, in vivo cDNA synthesis is expected to be a broadly enabling technology, with applications in cDNA synthesis, cDNA cloning, sample preparation and stabilization, gene expression analysis and gene discovery, and molecular diagnostics.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43CA081870-01
Application #
2869157
Study Section
Special Emphasis Panel (ZCA1-SRRB-C (J1))
Program Officer
Heath, Anne K
Project Start
1999-04-13
Project End
2001-03-31
Budget Start
1999-04-13
Budget End
2000-03-31
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Ivs Technologies, LLC
Department
Type
DUNS #
City
Carlsbad
State
CA
Country
United States
Zip Code
92009