A stable, versatile, nonpeptide synthetic peroxidase enzyme mimic will be prepared in the proposed phase I research. The design of the catalyst is based on the well-precedented peroxidase activity of synthetic metalloporphyrins. This catalyst will be readily soluble in organic solvents and will be equipped with functional group handles which will allow aqueous-so lubilizing groups to be attached as well. Conjugation to antibodies and other proteins and to solid phase supports will be demonstrated. The structural aspects of the catalyst have been designed to overcome the limitations of natural peroxidase enzymes by providing better stability, better resistance to inactivation, better resistance to autooxidation, better diffusion characteristics, better accessibility to transduction, and better conjugation features. The improved catalyst will be directly applicable to ELISA and other enzyme-based analytical techniques both as an improved replacement for current reporter peroxidases and as a new innovation to allow organic phase immunoassay development.

Proposed Commercial Applications

The proposed catalyst could replace horseradish peroxidase in aqueous ELISA applications. In addition, HBG holds a patent on the use of antibodies in organic phase. Organic phase immunoassay applications utilizing the catalyst would be immediately valuable for detecting environmental contaminants such as polychlorinated aromatics, insecticides, agrochemicals, and others, for which current assay methods are extremely expensive due to labor intensive sample preparation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43GM055499-01
Application #
2024059
Study Section
Special Emphasis Panel (ZRG2-SSS-4 (02))
Project Start
1997-02-01
Project End
1998-11-30
Budget Start
1997-02-01
Budget End
1998-11-30
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Hawaii Biotech, Inc.
Department
Type
DUNS #
113226823
City
Aiea
State
HI
Country
United States
Zip Code
96701