This is a Phase II SBIR proposal to detect fungal growth and anti-fungal drug sensitivity using One Cell System's gel microdrop encapsulation technology (GMD) combined with flow cytometry (FACS) analysis and cell sorting. Phase I studies will be expanded to evaluate additional potentially pathogenic fungi. Results obtained using model cell lines will further be compared with results using clinical isolates of several pathogenic fungal strains. In addition, GMD will be integrated with nucleic acid probe identification and amplification models. This rapid and sensitive assay will impact clinical diagnostics and drug discovery by improving antifungal susceptibility testing and permitting recovery of microorganisms of interest for subsequent cloning or gene sequence analysis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
5R44AI039295-03
Application #
2887137
Study Section
Special Emphasis Panel (ZRG2-SSS-3 (51))
Program Officer
Dixon (Dmid), Dennis M
Project Start
1996-09-30
Project End
2000-05-31
Budget Start
1999-06-01
Budget End
2000-05-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost
Name
One Cell Systems, Inc
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139