This proposal describes the development of semi-conductor nanocrystals (quantum dots) as probes for the specific detection of multiple markers of breast cell transformation simultaneously. Quantum dots are novel luminescent probes that are photo-stable, easily multiplexed and can be sensitively detected with simple, inexpensive optical systems. In phase I of the project we will use conjugated quantum dots to detect three selected breast cancer markers in sections of well-characterized breast cancer cell lines to demonstrate their specificity in a simple model system. In phase II we will multiplex this system, incorporate a fourth marker and develop it for use on tissues and tissue micro-arrays. We will detect the expression of three protein markers (p53, estrogen receptor and progesterone receptor). Initially we will characterize the assay specificity and performance of the probes individually. We will use this model to compare the stability and sensitivity of quantum dots with conventional fluorescent dyes. It is anticipated that this technology will allow the use of these probes to characterize breast tumor biopsies and ultimately quantum dots could be used to more confidently phenotype any disease for which multiple markers exist.

Proposed Commercial Applications

This research could directly lead to a novel product that allows more sensitive, quicker and more informative diagnostic and prognostic evaluation of breast cancer tissue sections. The technology is also directly applicable to many different kinds of tumor analysis and could be used on tissue microarrays to vastly increase the throughput and aid the archiving of tissue section analysis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
1R44CA088391-01
Application #
6203903
Study Section
Special Emphasis Panel (ZCA1-SRRB-C (M1))
Program Officer
Song, Min-Kyung H
Project Start
2000-08-28
Project End
2001-02-28
Budget Start
2000-08-28
Budget End
2001-02-28
Support Year
1
Fiscal Year
2000
Total Cost
$98,000
Indirect Cost
Name
Quantum Dot Corporation
Department
Type
DUNS #
City
Hayward
State
CA
Country
United States
Zip Code
94545
Ouzounov, Dimitre G; Ahmad, Faisal R; Muller, Dirk et al. (2003) Generation of megawatt optical solitons in hollow-core photonic band-gap fibers. Science 301:1702-4
Wu, Xingyong; Liu, Hongjian; Liu, Jianquan et al. (2003) Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat Biotechnol 21:41-6
Larson, Daniel R; Zipfel, Warren R; Williams, Rebecca M et al. (2003) Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 300:1434-6