The principal objective of this application is to establish a new methodology for the early detection of abnormal lesions, whether pre-cancerous or cancerous, in breast imaging. Early detection is crucial to identifying lesions while they are still contained when the cure rate is near 100%. Existing mammography often does not identify lesions until significant growth has occurred. Because of the importance of the problem, there is research in alternative methods, including MRI, ultrasound, scintigraphy, and other methods. We propose here, a novel approach using biomagnetic sensors and targeted superparamagnetic nanoparticles. The sensor will detect and image lesions in-vivo to which these particles have bound by antibodies or angiogenesis agents after injection into the subject. We have demonstrated in Phase I of this program, that Superconducting Quantum Interference Devices (SQUID) are capable of detecting sub- nanograms of these nanoparticles. This methodology has advantages over existing mammography in the flexibility of nanoparticle choices to increase specificity through antibodies targeting cancer types such as HER-2, and angiogenesis agents targeting tumor microvascular structure. Nanoparticles will also be used to image sentinel cells for metastases. We will use phantoms and tissue preparations to characterize nanoparticles for sensitivity and image capabilities and animal studies to verify the methodology. We will further affirm imaging accuracy through the use of these nanoparticles as contrast agents in MRI studies in our animal studies. We anticipate that the detection of lesions by magnetic sensors will provide a lower false positive rate than mammograms and, more importantly, a higher true positive rate. ? ?

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
2R44CA096154-02A1
Application #
7050751
Study Section
Special Emphasis Panel (ZRG1-SBIB-H (13))
Program Officer
Baker, Houston
Project Start
2002-04-29
Project End
2009-08-31
Budget Start
2006-09-20
Budget End
2007-08-31
Support Year
2
Fiscal Year
2006
Total Cost
$634,225
Indirect Cost
Name
Senior Scientific
Department
Type
DUNS #
072409886
City
Albuquerque
State
NM
Country
United States
Zip Code
87111
Huang, Ming-Xiong; Anderson, Bill; Huang, Charles W et al. (2017) Development of advanced signal processing and source imaging methods for superparamagnetic relaxometry. Phys Med Biol 62:734-757
Adolphi, Natalie L; Butler, Kimberly S; Lovato, Debbie M et al. (2012) Imaging of Her2-targeted magnetic nanoparticles for breast cancer detection: comparison of SQUID-detected magnetic relaxometry and MRI. Contrast Media Mol Imaging 7:308-19
Johnson, Cort; Adolphi, Natalie L; Butler, Kimberly L et al. (2012) Magnetic Relaxometry with an Atomic Magnetometer and SQUID Sensors on Targeted Cancer Cells. J Magn Magn Mater 324:2613-2619
Bryant, H C; Adolphi, Natalie L; Huber, Dale L et al. (2011) Magnetic Properties of Nanoparticles Useful for SQUID Relaxometry in Biomedical Applications. J Magn Magn Mater 323:767-774
Hathaway, Helen J; Butler, Kimberly S; Adolphi, Natalie L et al. (2011) Detection of breast cancer cells using targeted magnetic nanoparticles and ultra-sensitive magnetic field sensors. Breast Cancer Res 13:R108
Adolphi, Natalie L; Huber, Dale L; Bryant, Howard C et al. (2010) Characterization of single-core magnetite nanoparticles for magnetic imaging by SQUID relaxometry. Phys Med Biol 55:5985-6003
Adolphi, Natalie L; Huber, Dale L; Jaetao, Jason E et al. (2009) Characterization of magnetite nanoparticles for SQUID-relaxometry and magnetic needle biopsy. J Magn Magn Mater 321:1459-1464
Flynn, E R; Bryant, H C (2005) A biomagnetic system for in vivo cancer imaging. Phys Med Biol 50:1273-93