This proposal aims to develop a robust and ultra high-throughput technology for simultaneously assaying methylation at many specific sites in a genome. The technology we aim to develop will be based on a beadbased optical fiber array platform. This system is capable of analyzing hundreds to thousands of samples per day, and performing more than a million methylation measurements per instrument per day.
We aim to provide a system that will enable high-resolution methylation profiling in large numbers of samples. The technology developed in this proposal will provide a powerful tool not only for fundamental genomic research, but also for cancer biologystudies, with potential application to cancer classification and diagnosis, and anti-cancer drug target identification and drug screening. ? ? ? ?
| Lin, Zhenwu; Thomas, Neal J; Bibikova, Marina et al. (2007) DNA methylation markers of surfactant proteins in lung cancer. Int J Oncol 31:181-91 |
| Bibikova, Marina; Lin, Zhenwu; Zhou, Lixin et al. (2006) High-throughput DNA methylation profiling using universal bead arrays. Genome Res 16:383-93 |
| Bibikova, Marina; Chudin, Eugene; Wu, Bonnie et al. (2006) Human embryonic stem cells have a unique epigenetic signature. Genome Res 16:1075-83 |
