The main goal of the project is to increase the sensitivity of microtransponder-based multiplexed bioassays by (1) introducing a coating with silver nanostructures onto the microtransponders (MTPs) to enhance fluorescence emission, and (2) improving the sensitivity of fluorescence detection of the analyzer. The key element of the assay is the MTP, a monolithic 500 W 500 The proposed system for an ultra-sensitive assay provides a new tool for performing several tests at once. The system is applicable to cancer diagnostics and other medical ailments providing a more powerful, simpler to use and less costly approach. An assay will be developed to diagnose prostate cancer by testing urine in an non-invasive way at early phases of the disease. The assay has a potential to also improve PCa disease management. Acceptance of nanotechnology-based multiplex assays on microtransponders by researchers and clinicians could initiate new methods in disease diagnosis and treatment, drug discovery, and biological or environmental research.Public Health Relevance
| Rich, Ryan; Li, Ji; Fudala, Rafal et al. (2012) Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays. Anal Bioanal Chem 404:2223-31 |
| Li, Ji; Wang, Zhuying; Gryczynski, Ignacy et al. (2010) Silver nanoparticle-enhanced fluorescence in microtransponder-based immuno- and DNA hybridization assays. Anal Bioanal Chem 398:1993-2001 |
