Abstract

Chromosome-specific DNAs will be prepared for all mouse and rat chromosomes by combining the technologies of chromosome microdissection and degenerate oligonucleotide primed PCR (DOP-PCR) amplification. Difficulties with flow sorting of chromosomes have prevented complete sets of chromosome-specific DNAs or libraries other than human from being made available. Microdissection will circumvent these difficulties and allow pure chromosome-specific DNAs to be prepared from all chromosomes. A single degenerate primer will allow amplification of DNA from both mouse and rat. Cot1 DNA for use as competitor in hybridizations will be prepared for both species. Chromosome-specific DNAs will be tested for specificity by painting. Selected DNAs will be tested for representation by hybridization to a battery of unique chromosome-specific probes. Chromosome-specific DNAs will be cloned using conventional techniques. Chromosome-specific DNAs, Cot1 DNA, and protocols for use will be made available to the research community.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
9R44ES008020-02
Application #
2157488
Study Section
Special Emphasis Panel (ZRG7-SSS-2 (02))
Project Start
1993-08-16
Project End
1997-09-29
Budget Start
1995-09-30
Budget End
1996-09-29
Support Year
2
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
Applied Genetics Laboratories, Inc.
Department
Type
DUNS #
City
Melbourne
State
FL
Country
United States
Zip Code
32904