This research project is aimed at developing a novel DNA sequencing, mapping, or diagnostic technique that requires no DNA labeling. The proposed innovative technique would even allow genomic DNA to be used for diagnostics, thus significantly reducing preparation cost (no polymerase chain reaction would be necessary). During Phase l we experimentally evaluated the most promising detection techniques and demonstrated the feasibility of the procedure. In Phase Il we plan to systematically determine the best parameters for probe immobilization and hybridization and determine the sensitivity of these procedures to variations in the parameters. We will also develop a less expensive prototype instrument that could be purchased by major facilities. Because of the potential cost effectiveness, accuracy and speed of this innovative technique, commercial benefits could be tremendous.

Proposed Commercial Applications

Commercialization could begin by supplying analytical services to researchers at national and private laboratories. Instrument sales would be first targeted to major research and clinical laboratories, but would grow to areas outside the biomedical field due to the wide applicability of the technique.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
5R44HG001596-04
Application #
6164971
Study Section
Special Emphasis Panel (ZRG2-GNM (02))
Program Officer
Couch, Jennifer A
Project Start
1997-04-01
Project End
2002-01-31
Budget Start
2000-03-01
Budget End
2002-01-31
Support Year
4
Fiscal Year
2000
Total Cost
$133,783
Indirect Cost
Name
Atom Sciences, Inc.
Department
Type
DUNS #
048900880
City
Oak Ridge
State
TN
Country
United States
Zip Code
37830