This Fast-Track SBIR proposal is directed toward the development of new technology for high-throughput, low cost, single nucleotide polymorphism (SNP) genotyping. The investigators predict that high-resolution SNP maps will revolutionize the science and practice of genetics. The new technology will make use of self-assembled arrays of beads on optical fibers, because of the rapidity of self-assembly and its flexibility for customization of arrays and their manufacture on demand. These arrays have 10-fold more elements per unit area than the most densely packed conventional arrays and densities can be increased further.
The specific aims i n Phase I relate to determining the identity of beads bearing oligonucleotide probes at each position in a randomly ordered array. The feasibility of performing this decoding procedure will be tested in a model system consisting of 16 different probes-on-beads. If Phase I is successful, an SNP genotyping system with a projected capacity of 3,000,000 genotypes per assay cycle will be developed in Phase II.
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| Gunderson, Kevin L; Kruglyak, Semyon; Graige, Michael S et al. (2004) Decoding randomly ordered DNA arrays. Genome Res 14:870-7 |
| Fan, J B; Oliphant, A; Shen, R et al. (2003) Highly parallel SNP genotyping. Cold Spring Harb Symp Quant Biol 68:69-78 |
| Oliphant, Arnold; Barker, David L; Stuelpnagel, John R et al. (2002) BeadArray technology: enabling an accurate, cost-effective approach to high-throughput genotyping. Biotechniques Suppl:56-8, 60-1 |
