Millions of people in the USA and throughout the world are affected by neurological disorders such as epilepsy, Parkinson's disease and brain tumors. Confocal microscopy has considerably enhanced the testing of hypotheses in the basic neurosciences, providing significant insights into the causes of such neurological disorders. However, these systems are currently extremely expensive, putting them out of the reach of most individual scientists; the majority of confocal microscopes are currently to be found only in core facilities. There is also a need for increased optical resolution and the ability to perform 3-dimensional reconstructions of samples using a confocal microscope. If a system could be brought to market at a cost of less than $50,000, confocal microscopy would become available to every individual scientist using fluorescence microscopy, thus improving research abilities and potentially alleviating the financial and medical burdens caused by neurological disorders. ? ? The overall goal of this project is to develop a new type of confocal microscope based on an innovative concept - the swept-field confocal design (SFC). The SFC is a revolutionary confocal concept. This hybrid-instrument uses an array of stationary pinholes for illumination and detection along one axis while using a single galvanometer to sweep the illumination along the other axis (Patent Pending). In Phase I of this project we designed, built prototypes of and evaluated the performance of two variations of the SFC. In Phase II of this project we will move from the drawing board to the laboratory; we will develop, test and refine a high-performance, easy-to-use swept-field confocal microscope at a selling price of under $50,000. ? ?
| Davenport, Nicholas R; Sonnemann, Kevin J; Eliceiri, Kevin W et al. (2016) Membrane dynamics during cellular wound repair. Mol Biol Cell 27:2272-85 |
| Ponomareva, Olga Y; Eliceiri, Kevin W; Halloran, Mary C (2016) Charcot-Marie-Tooth 2b associated Rab7 mutations cause axon growth and guidance defects during vertebrate sensory neuron development. Neural Dev 11:2 |
| Ponomareva, Olga Y; Holmen, Ian C; Sperry, Aiden J et al. (2014) Calsyntenin-1 regulates axon branching and endosomal trafficking during sensory neuron development in vivo. J Neurosci 34:9235-48 |
| Bembenek, Joshua N; Richie, Christopher T; Squirrell, Jayne M et al. (2007) Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase. Development 134:3837-48 |
