Using new instrumentation for imaging biological fine structure, contact x-ray microscopy coupled to transmission electron microscopy will be used to examine a variety of tissues and cells. A prototype instrument designed at the IBM Thomas Watson Research Center will be used. Biological specimens prepared by a variety of procedures such as conventional fixation; fresh frozen ultracryomicrotomy sections; air dried or critical point dried whole cells will be compared following exposure of these plastic resists to a soft x-ray source. Adaptation of this mode of imaging will test this new instrumentation as to potentially wider applicability and possibly provide insight into hitherto unrevealed organization of sub-cellular components.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Unknown (S03)
Project #
2S03RR003116-04
Application #
3510785
Study Section
(NSS)
Project Start
1987-03-01
Project End
1988-09-15
Budget Start
1987-03-01
Budget End
1988-09-15
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Suny Downstate Medical Center
Department
Type
Schools of Medicine
DUNS #
068552207
City
Brooklyn
State
NY
Country
United States
Zip Code
11203