Abstract

The present research project is related to the influence of plasma and cardiac renin-angiotensin system on the regulation of cell communication in the heart. My major aim is to understand the role of the cardiac renin- angiotensin system on the process of gap junction communication as well as on impulse propagation. Heart cell pairs will be obtained by enzymatic dispersion of adult rat or hamster ventricle. To determine the junctional conductance two isolated voltage clamp systems will be used. Giga-ohm scaling will be obtained and then th cell membrane of both cells of the pair will be broken by stronger suction. The holding potential of both cells will be .40 mV. Cell 1 will be then pulsed to O mV while membrane potential of cell 2 will be kept unchanges. The junctional resistance (or conductance) will be then determined with values of junctional current and transjunctional voltage. Action potentials will be recorded from intact papillary muscles or Parkinje fiber with intracellular electrodes. It is known that the cardiac renin-angiotensin system is activated during congestive heart failure or myocardial infarction but the real meaning of this phenomenon is not known. The present research project is involved with the study of this problem and will certainly provide important information on the role of this system on cell communication.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
2S06GM008224-12
Application #
5211921
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Rijpma, Sanna R; van der Velden, Maarten; González-Pons, Maria et al. (2016) Multidrug ATP-binding cassette transporters are essential for hepatic development of Plasmodium sporozoites. Cell Microbiol 18:369-83
Padín-Irizarry, Vivian; Colón-Lorenzo, Emilee E; Vega-Rodríguez, Joel et al. (2016) Glutathione-deficient Plasmodium berghei parasites exhibit growth delay and nuclear DNA damage. Free Radic Biol Med 95:43-54
Jardón, Javier; Izquierdo, Natalio J; Renta, Jessica Y et al. (2016) Ocular Findings in Patients with the Hermansky-Pudlak Syndrome (Types 1 and 3). Ophthalmic Genet 37:89-94
Rivera-Peña, Bianca; Ruíz-Fullana, Francisco J; Vélez-Reyes, Germán L et al. (2016) HPV-16 infection modifies overall survival of Puerto Rican HNSCC patients. Infect Agent Cancer 11:47
Velásquez-Martínez, Maria C; Vázquez-Torres, Rafael; Rojas, Legier V et al. (2015) Alpha-1 adrenoreceptors modulate GABA release onto ventral tegmental area dopamine neurons. Neuropharmacology 88:110-21
Vega-Rodríguez, Joel; Pastrana-Mena, Rebecca; Crespo-Lladó, Keila N et al. (2015) Implications of Glutathione Levels in the Plasmodium berghei Response to Chloroquine and Artemisinin. PLoS One 10:e0128212
Zenón, Frances; Cantres-Rosario, Yisel; Adiga, Radhika et al. (2015) HIV-infected microglia mediate cathepsin B-induced neurotoxicity. J Neurovirol 21:544-58
Rosas, Odrick R; Torrado, Aranza I; Santiago, Jose M et al. (2014) Long-term treatment with PP2 after spinal cord injury resulted in functional locomotor recovery and increased spared tissue. Neural Regen Res 9:2164-73
Mosquera, Laurivette; Colón, Jennifer M; Santiago, José M et al. (2014) Tamoxifen and estradiol improved locomotor function and increased spared tissue in rats after spinal cord injury: their antioxidant effect and role of estrogen receptor alpha. Brain Res 1561:11-22
Hodakoski, Cindy; Hopkins, Benjamin D; Barrows, Douglas et al. (2014) Regulation of PTEN inhibition by the pleckstrin homology domain of P-REX2 during insulin signaling and glucose homeostasis. Proc Natl Acad Sci U S A 111:155-60

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