The goal of this NIH shared instrumentation application is to establish an integrated, state-of-the-art, DNA sequencing core system at the Buck Institute to facilitate ongoing and future scientific research on the molecular mechanisms of aging and age-related diseases. The latest technology has developed new capillary electrophoresis sequencing instrumentation to allow high quality, rapid DNA sequencing to be achieved in a completely automated format that utilizes sensitive fluorescent dye terminators. These features of the ABI 3100 Genetic Analyzer System for capillary electrophoresis system are advantageous over conventional slab gel dye terminator DNA sequencing that is used by most other DNA sequencing facilities. The automation of capillary preparation, sample loading, and electrophoresis significantly improves clarity and consistency of results; the automated format avoids operator errors that occur with the manual slab gel format of conventional DNA sequencers. The integrated DNA sequencing system will include the Expedite 8909 oligonucleotide synthesizer for synthesis of primers needed for the high volume of DNA sequencing; this unit also has the unique capability for synthesizing peptide nucleic acids (PNA) that are especially effective for antisense gene expression, as well as synthesis of modified and unmodified oligonucleotides. This integrated DNA sequencing system will enhance ongoing NIH-funded research (and research funded by other sources) for principal investigators at the Buck Institute, will complement existing cores in genomics, proteomics, and morphology and cell biology, and will foster collaborations among investigators at the Institute.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
7S10RR016614-02
Application #
6894499
Study Section
Special Emphasis Panel (ZRG1-SSS-G (01))
Program Officer
Tingle, Marjorie
Project Start
2004-06-01
Project End
2005-07-14
Budget Start
2004-06-01
Budget End
2005-07-14
Support Year
2
Fiscal Year
2002
Total Cost
$51,189
Indirect Cost
Name
University of California San Diego
Department
Type
Schools of Pharmacy
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Ziegler, Micah S; Levine, Daniel S; Lakshmi, K V et al. (2016) Aryl Group Transfer from Tetraarylborato Anions to an Electrophilic Dicopper(I) Center and Mixed-Valence ?-Aryl Dicopper(I,II) Complexes. J Am Chem Soc 138:6484-91
Lomri, N; Thomas, J; Cashman, J R (1993) Expression in Escherichia coli of the cloned flavin-containing monooxygenase from pig liver. J Biol Chem 268:5048-54
Cashman, J R; Celestial, J R; Leach, A et al. (1993) Tertiary amines related to brompheniramine: preferred conformations for N-oxygenation by the hog liver flavin-containing monooxygenase. Pharm Res 10:1097-105
Cashman, J R; Celestial, J R; Leach, A R (1992) Enantioselective N-oxygenation of chlorpheniramine by the flavin-containing monooxygenase from hog liver. Xenobiotica 22:459-69
Yang, Z C; Cashman, J R (1992) Structure-retention index relationships for derivatized monosaccharides on non-polar gas chromatography columns. J Chromatogr 596:79-84
Chamberlain, J G; Dittmann, L E; Hunt, S et al. (1991) Effects of long term 2% fish oil supplements on tissue fatty acids, phospholipids, cholesterol, and arterial histology in Japanese quail (Coturnix coturnix japonica). Artery 18:291-314
Cashman, J R; Liu, Y (1991) Electron-deficient isoalloxazines: model systems for disulfide prodrug formation. J Med Chem 34:2049-55
Cashman, J R; Olsen, L D (1990) Stereoselective S-oxygenation of 2-aryl-1,3-dithiolanes by the flavin-containing and cytochrome P-450 monooxygenases. Mol Pharmacol 38:573-85
Cashman, J R; Olsen, L D; Bornheim, L M (1990) Enantioselective S-oxygenation by flavin-containing and cytochrome P-450 monooxygenases. Chem Res Toxicol 3:344-9
Cashman, J R; Williams, D E (1990) Enantioselective S-oxygenation of 2-aryl-1,3-dithiolanes by rabbit lung enzyme preparations. Mol Pharmacol 37:333-9

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