We aim to develop an innovative approach to generate, at high-throughput, isogenic induced pluripotent stem cells (iPSCs), and use their differentiated progeny to understand the impact of human genetic variation on the risk of developing coronary artery disease (CAD). A genomic variant associated with CAD, myocardial infarction (Ml), abdominal aortic aneurysm, and intracranial aneurysm is found in a stretch of chr9p21 devoid of known genes. We will use this locus as a model for our study; while focusing on 9p21, our overall approach will be broadly applicable to the study of HLBS diseases of complex genetic architecture.
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| Lo Sardo, Valentina; Ferguson, William; Erikson, Galina A et al. (2017) Influence of donor age on induced pluripotent stem cells. Nat Biotechnol 35:69-74 |
| Yu, Chen; Liu, Yanxia; Ma, Tianhua et al. (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16:142-7 |
| Yu, Chen; Liu, Kai; Tang, Shibing et al. (2014) Chemical approaches to cell reprogramming. Curr Opin Genet Dev 28:50-56 |
| Zhang, Yu; Li, Wenlin; Laurent, Timothy et al. (2012) Small molecules, big roles -- the chemical manipulation of stem cell fate and somatic cell reprogramming. J Cell Sci 125:5609-20 |
