The long-range goal of the Neuroscience Microscopy and Immunohistochemistry Core is to provide a stateof- the-art facility that will enable neuroscience investigators to correlate nervous system structure and function. Neuroscientists at Meharry Medical College are exploring various aspects of how the nervous system responds to environmental and internal stressors that may induce inflammatory processes and neuro-degeneration. Of particular interest is the question of how genes and the environment interact to cause neurodegenerative disease.
The specific aims of the Neuroscience Microscopy and immunohistochemistry Core will be to: 1. Provide neuro-morphological services that will accommodate the needs of greater than 80% of the neuroscience laboratories at Meharry, thereby decreasing the turn-around time associated with using a remote facility; 2. Decrease by 20% the costs associated with doing morphological studies in the nervous system: and 3. Insure that Meharry Medical College investigators have e-journal access to the top 10 journals that publish neuroscience research results. Dr. Clivel G. Charlton, Chair of the Division of Neurobiology and Neurotoxicology will direct the Neuroscience Microscopy and Immunohistochemistry facility. A trained Ph.D. level Core Director will be recruited for the day-to-day management of the facility. The Core Director will also implement an ongoing training program for investigators, research staff and trainees. As a result of the recent re-organization of the basic science departments, a number of microscopic and cell/tissue processing resource are available to initiate this core facility. This includes a new confocal microscope, a cyrostat, microtome, and routine histochemistry supplies. There is the need to acquire a new fluorescence microscope, image analysis software and enhance the neuroscience e-journal collection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01NS041071-10
Application #
8146201
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
2013-08-31
Budget Start
2010-09-01
Budget End
2011-08-31
Support Year
10
Fiscal Year
2010
Total Cost
$236,250
Indirect Cost
Name
Meharry Medical College
Department
Type
DUNS #
041438185
City
Nashville
State
TN
Country
United States
Zip Code
37208
Hamilton, Vanae; Singha, Ujjal K; Smith, Joseph T et al. (2014) Trypanosome alternative oxidase possesses both an N-terminal and internal mitochondrial targeting signal. Eukaryot Cell 13:539-47
Smith, Marquitta L; King, Jennifer; Dent, Lemuel et al. (2014) Effects of acute and sub-chronic L-dopa therapy on striatal L-dopa methylation and dopamine oxidation in an MPTP mouse model of Parkinsons disease. Life Sci 110:1-7
Ferguson, Marcus C; Nayyar, Tultul; Ansah, Twum A (2014) Reverse microdialysis of a 5-HT2A receptor antagonist alters extracellular glutamate levels in the striatum of the MPTP mouse model of Parkinson's disease. Neurochem Int 71:36-46
Malloy, Melanie Theodore; McIntosh, Deneshia J; Walters, Treniqka S et al. (2013) Trafficking of the transcription factor Nrf2 to promyelocytic leukemia-nuclear bodies: implications for degradation of NRF2 in the nucleus. J Biol Chem 288:14569-83
Duncan, Melanie R; Fullerton, Marjorie; Chaudhuri, Minu (2013) Tim50 in Trypanosoma brucei possesses a dual specificity phosphatase activity and is critical for mitochondrial protein import. J Biol Chem 288:3184-97
Lu, Wenfu; Xie, Yingqiu; Ma, Yufang et al. (2013) ARF represses androgen receptor transactivation in prostate cancer. Mol Endocrinol 27:635-48
Bailey, Charvann K; Mittal, Mukul K; Misra, Smita et al. (2012) High motility of triple-negative breast cancer cells is due to repression of plakoglobin gene by metastasis modulator protein SLUG. J Biol Chem 287:19472-86
Mackey, Veronica R; Muthian, Gladson; Smith, Marquitta et al. (2012) Prenatal exposure to methanol as a dopamine system sensitization model in C57BL/6J mice. Life Sci 91:921-7
King, Jennifer M; Muthian, Gladson; Mackey, Veronica et al. (2011) L-Dihydroxyphenylalanine modulates the steady-state expression of mouse striatal tyrosine hydroxylase, aromatic L-amino acid decarboxylase, dopamine and its metabolites in an MPTP mouse model of Parkinson's disease. Life Sci 89:638-43
Ansah, Twum A; Ferguson, Marcus C; Nayyar, Tultul et al. (2011) Age- and duration-dependent effects of MPTP on cortical serotonin systems. Neurosci Lett 504:160-164

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