- CORE C: Single-Cell Analysis Core HR arises from cell-to-cell variation of genotypic and metabolic processes. The goal of the Single-Cell Analysis Core (Core C) is to provide experimentally-validated and yet difficult-to-perform single-cell-level analyses to evaluate the mechanisms and dynamics of HR. Core C will support the single-cell-level studies proposed in all three Projects by providing a centralized platform for culturing bacterial cells under well-controlled antibiotic environments, measuring relevant processes at single-cell resolution, and performing image analysis as well as necessary statistical analysis. Specifically, Core C will i) provide microfluidic continuous culture platform, ii) quantify the copy number variation of resistance genes, and iii) determine metabolic activities of single cells in heteroresistant populations. Core C activities will be conducted by Dr. Minsu Kim's laboratory in the Department of Physics at Emory University, which has extensive expertise in single-cell-level imaging and analysis of gene expression, metabolism, and physiology in bacteria. Dr. Kim was one of the first scientists to apply a single-cell approach to antibiotic research and is considered a leading expert in this area. Dr. Kim and his team will work with the PI of individual projects collaboratively to ensure they can successfully complete their projects in a timely manner.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AI158080-01
Application #
10170969
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2021-03-05
Project End
2026-02-28
Budget Start
2021-03-01
Budget End
2022-02-28
Support Year
1
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Emory University
Department
Type
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322