The Whitehead/MIT Center for Genome Research is eager to contribute to the goals of completing the sequence of the mouse genome in draft form by 2003 and in finished form by 2005. During the three-year project, we propose to produce sequence coverage of approximately 25 percent of the mouse genome. Specifically, we will sequence BAC clones totaling 750 Mb-consisting of 630 Mb of draft sequence at approximately 5 cent per base and 120 Mb of finished sequence at approximately 12 cent per base. Regions will be selected for sequencing on the bases of biological interest for the mouse community and for comparative mouse-human studies. In the course of the project, we also propose to (i) test strategies for genomic sequencing involving pooling of BAC clones and (ii) engage in technology transfer activities with new pilot centers.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
3U54HG002152-04S1
Application #
6468304
Study Section
Special Emphasis Panel (ZHG1 (O1))
Program Officer
Peterson, Jane
Project Start
1999-09-30
Project End
2003-10-31
Budget Start
2001-12-07
Budget End
2002-10-31
Support Year
4
Fiscal Year
2002
Total Cost
$4,323,000
Indirect Cost
Name
Whitehead Institute for Biomedical Research
Department
Type
DUNS #
076580745
City
Cambridge
State
MA
Country
United States
Zip Code
02142
Thompson, E E; Kuttab-Boulos, H; Yang, L et al. (2006) Sequence diversity and haplotype structure at the human CYP3A cluster. Pharmacogenomics J 6:105-14
Kudo, Mariko; Bao, Ming; D'Souza, Anil et al. (2005) The alpha- and beta-subunits of the human UDP-N-acetylglucosamine:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase [corrected] are encoded by a single cDNA. J Biol Chem 280:36141-9