The full extent of myocardial contractile recovery following ischemia is delayed beyond the immediate reperfusion (R). This may be associated with delayed metabolic recovery and/or CA2+ overload. This can be quantified in the beating heart by the scattered laser intensity fluctuations (SLIF) which sense Ca oscillations (CaOs) it produces. We measured ATP, and pH by 31p NMR and SLIF, and developed pressure in isovolumic rat hearts. During the first 5 minute of R there is rapid metabolic but a more gradual developed pressure and SLIF recovery. The both gradually recover developed pressure abruptly and SLIF increases fourfold over the next 40 minute mean no change in metabolic parameters. These findings suggest that delayed contractile recovery during R is attributable in part to and adverse effect of increased CaOs induced by Ca2+ overload during R. In additional hearts, for the first 10 minutes of R, perfusate Ca was 1.5mM (Group 1, N=4) or 0.08mM (Group 2, n=4). During R the early peak in SLIF was not present in Group 2. Recovery of DP was higher and cell Ca lower in Group 2 vs Group 1 at 20 minute R. Thus, CaOsc during early R are modified by perfusate Ca, noninvasively index the extent of Ca loading, and predict functional recovery; Other hearts were pretreated with Amiloride 10-4, a known Na- Ca blocker during hypoxia and reoxygenation (ReO2). During ReO2, DP, EDP and PCr (all % baseline), and pHi recovery were improved in the Amiloride treated hearts. Cell gain during ReO2 was reduced 50% by Amiloride. These results suggest that cell Ca loading during reoxygenation is Na dependent and may be mediated in part by Na/Ca exchange.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000244-02
Application #
3821464
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Mynard, Jonathan P; Wasserman, Bruce A; Steinman, David A (2013) Errors in the estimation of wall shear stress by maximum Doppler velocity. Atherosclerosis 227:259-66
Hoi, Yiemeng; Wasserman, Bruce A; Lakatta, Edward G et al. (2010) Carotid bifurcation hemodynamics in older adults: effect of measured versus assumed flow waveform. J Biomech Eng 132:071006
Hoi, Yiemeng; Wasserman, Bruce A; Xie, Yuanyuan J et al. (2010) Characterization of volumetric flow rate waveforms at the carotid bifurcations of older adults. Physiol Meas 31:291-302