Abstract

1. Using cultured neurons, we manipulated intracellular sodium concentrations as a marker of activity. Increased intracellular sodium caused by monensin stimulated the sodium pump to consume ATP. Immediately thereafter, transcription of mitochondrial-derived enzymes for oxidative phosphorylation was upregulated, which would lead to increased ATP synthesis. This study identified the rapid coupling mechanism between electrical activity, ATP consumption and oxidative-phosphorylation activation in mitochondria for new ATP synthesis. (Mehrabian et al 2005). 2. As a model for heart sympathetic denervation in Parkinson disease, rats were sympathetically denervated with 6-hydroxydopamine (6-OHDA). Extending our in vivo model for the brain, we measured incorporation of palmitic and arachidonic acids into phospholipids of denervated and normal hearts. Incorporation of palmitic acid (from the acyl-CoA precursor pool) was unchanged, whereas incorporation of arachidonic acid was reduced by 70%, accompanied by a reduction in phospholipase A2 activity. It now would be of interest to see if Parkinson disease patients have this denervation defect, using positron emission tomography. (Patrick et al 2005)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000399-01
Application #
7132265
Study Section
(BPM)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2005
Total Cost
Indirect Cost

  Institution

Name
Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Krasemann, Susanne; Madore, Charlotte; Cialic, Ron et al. (2017) The TREM2-APOE Pathway Drives the Transcriptional Phenotype of Dysfunctional Microglia in Neurodegenerative Diseases. Immunity 47:566-581.e9
Rapoport, Stanley I (2013) Translational studies on regulation of brain docosahexaenoic acid (DHA) metabolism in vivo. Prostaglandins Leukot Essent Fatty Acids 88:79-85
Yuan, Zhi-Xin; Rapoport, Stanley I; Soldin, Steven J et al. (2013) Identification and profiling of targeted oxidized linoleic acid metabolites in rat plasma by quadrupole time-of-flight mass spectrometry. Biomed Chromatogr 27:422-32
Kim, Hyung-Wook; Rao, Jagadeesh S; Rapoport, Stanley I et al. (2011) Regulation of rat brain polyunsaturated fatty acid (PUFA) metabolism during graded dietary n-3 PUFA deprivation. Prostaglandins Leukot Essent Fatty Acids 85:361-8
Gao, Fei; Kiesewetter, Dale; Chang, Lisa et al. (2009) Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified alpha-linolenic acid in unanesthetized rats. J Lipid Res 50:749-58
Igarashi, Miki; DeMar Jr, James C; Ma, Kaizong et al. (2007) Docosahexaenoic acid synthesis from alpha-linolenic acid by rat brain is unaffected by dietary n-3 PUFA deprivation. J Lipid Res 48:1150-8
Rao, J S; Ertley, R N; DeMar Jr, J C et al. (2007) Dietary n-3 PUFA deprivation alters expression of enzymes of the arachidonic and docosahexaenoic acid cascades in rat frontal cortex. Mol Psychiatry 12:151-7
Rapoport, Stanley I; Rao, Jagadeesh S; Igarashi, Miki (2007) Brain metabolism of nutritionally essential polyunsaturated fatty acids depends on both the diet and the liver. Prostaglandins Leukot Essent Fatty Acids 77:251-61
Igarashi, Miki; Ma, Kaizong; Chang, Lisa et al. (2007) Dietary n-3 PUFA deprivation for 15 weeks upregulates elongase and desaturase expression in rat liver but not brain. J Lipid Res 48:2463-70
Igarashi, Miki; DeMar Jr, James C; Ma, Kaizong et al. (2007) Upregulated liver conversion of alpha-linolenic acid to docosahexaenoic acid in rats on a 15 week n-3 PUFA-deficient diet. J Lipid Res 48:152-64

Showing the most recent 10 out of 15 publications