Abstract

Previously, we found that chromatin remodeling was required for Th2 cytokine gene expression. As the remodeling enzyme bound to the affected loci, the role of the remodeling enzyme appears to be direct.? ? Progress:? ? We identified a transcription factor that associates with a remodeling enzyme in a cytokine-dependent manner.? ? We identified two transcription factors that recruit a remodeling enzyme to the Th2 locus.? ? We identified a transcription factor that facilitates binding of another transcription factor to the Th2 locus.? ? We identified the version of a remodeling complex that regulates Th2 gene expression and chromatin structure.? ? We identified another remodeling enzyme regulating activation-inducible genes in a T cell line.? ? Future: ? Remodeling and other cytokine loci.? Remodeling and other T cell fates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000524-04
Application #
7732252
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2008
Total Cost
$438,713
Indirect Cost

  Institution

Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Wurster, Andrea L; Pazin, Michael J (2012) ATP-dependent chromatin remodeling in T cells. Biochem Cell Biol 90:1-13
Honda, Hiroshi; Pazin, Michael J; Ji, Hongxiu et al. (2006) Crucial roles of Sp1 and epigenetic modifications in the regulation of the CLDN4 promoter in ovarian cancer cells. J Biol Chem 281:21433-44
Shogren-Knaak, Michael; Ishii, Haruhiko; Sun, Jian-Min et al. (2006) Histone H4-K16 acetylation controls chromatin structure and protein interactions. Science 311:844-7
Lynch, Mary; Chen, Li; Ravitz, Michael J et al. (2005) hnRNP K binds a core polypyrimidine element in the eukaryotic translation initiation factor 4E (eIF4E) promoter, and its regulation of eIF4E contributes to neoplastic transformation. Mol Cell Biol 25:6436-53
Lu, Jun; Pazin, Michael J; Ravid, Katya (2004) Properties of ets-1 binding to chromatin and its effect on platelet factor 4 gene expression. Mol Cell Biol 24:428-41
Ishii, Haruhiko; Sen, Ranjan; Pazin, Michael J (2004) Combinatorial control of DNase I-hypersensitive site formation and erasure by immunoglobulin heavy chain enhancer-binding proteins. J Biol Chem 279:7331-8