The ATP-dependent Chromatin-remodeling complex, Rsc, was originally identified in yeast. It has similar subunit composition as SWI/SNF: 2 subunits are shared between the two complexes and at least 4 others are homologues of each other. However, the function of Rsc is distinct from SWI/SNF. Rsc is essential to the mitotic growth of yeast, whereas SWI/SNF is not. The rsc mutants are arrested at G2/M transition during the cell cycle and this arrest is dependent on the spindle-checkpoint gene. They are also more sensitive to microtubule-destabilizing drugs. These data suggest that Rsc may play a role in mitosis perhaps by stabilizing mitotic spindle formation. We have previously purified several ATP-dependent chromatin remodeling complexes from human which are closely-related to either yeast SWI/SNF or Rsc. By microsequencing and cloning, we now identified one subunit of a particular complex is a human homologue of yeast Rsc subunits, Rsc1 and Rsc2. We subsequently identified many other subunits of human Rsc complex and found many of them are identical to those in human SWI/SNF. Preliminary data suggest that human Rsc may directly participate in mitosis by binding to microtubules. We are continuing to investigate this part of the mechanism. - Chromatin, SWI/SNF, Rsc, transcription, mitosis

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000651-01
Application #
6227826
Study Section
Special Emphasis Panel (LG)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code