Smith has shown that when poly(A)+ RNA derived from human diploid fibroblasts at late passage at late passage is microinjected into the same cell type at early passage, the growth of the early passage cells is halted. A functionally identical activity derived from rat liver (by K. McClung and J. Smith) has a single molecular weight peak of activity on sucrose gradients. These data suggest that a single messenger RNA has the ability to shut off cell growth, and dilution experiments suggests that the message is abundant (1/100-1/1000 of total message). We plan to clone this mRNA by hybrid selection screening of a cDNA library, and then to analyze the structure and expression of the gene.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000705-03
Application #
3821516
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Sharov, Alexei A; Falco, Geppino; Piao, Yulan et al. (2008) Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary. BMC Biol 6:24
Zahn, Jacob M; Poosala, Suresh; Owen, Art B et al. (2007) AGEMAP: a gene expression database for aging in mice. PLoS Genet 3:e201