Abstract

We have studied DNA repair in individual primary rat neurons. The neurons repair many kinds of DNA damage, and it is particularly novel that they repair UV induced DNA damage. We find attenuation of oxidative DNA damage repair in differentiating neurons and we find that the DNA repair in the synaptic region is quite robust after oxidative stress. Furthermore, there seems to be a connection between neurotransmission and DNA repair because the addition of neurotransmitters to neurons incraeses the DNA damage and repair

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000723-01
Application #
7732296
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2008
Total Cost
$309,859
Indirect Cost

  Institution

Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Ghosh, Somnath; Canugovi, Chandrika; Yoon, Jeong Seon et al. (2015) Partial loss of the DNA repair scaffolding protein, Xrcc1, results in increased brain damage and reduced recovery from ischemic stroke in mice. Neurobiol Aging 36:2319-2330
Fang, Evandro Fei; Scheibye-Knudsen, Morten; Jahn, Heiko J et al. (2015) A research agenda for aging in China in the 21st century. Ageing Res Rev 24:197-205
Li, Xiaolin; Fang, Evandro Fei; Scheibye-Knudsen, Morten et al. (2014) Di-(2-ethylhexyl) phthalate inhibits DNA replication leading to hyperPARylation, SIRT1 attenuation, and mitochondrial dysfunction in the testis. Sci Rep 4:6434
Pan, Wen Liang; Wong, Jack Ho; Fang, Evandro Fei et al. (2014) Preferential cytotoxicity of the type I ribosome inactivating protein alpha-momorcharin on human nasopharyngeal carcinoma cells under normoxia and hypoxia. Biochem Pharmacol 89:329-39
Gredilla, Ricardo; Garm, Christian; Holm, Rikke et al. (2010) Differential age-related changes in mitochondrial DNA repair activities in mouse brain regions. Neurobiol Aging 31:993-1002
Weissman, Lior; de Souza-Pinto, Nadja C; Mattson, Mark P et al. (2009) DNA base excision repair activities in mouse models of Alzheimer's disease. Neurobiol Aging 30:2080-1
Maynard, Scott; Schurman, Shepherd H; Harboe, Charlotte et al. (2009) Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis 30:2-10
de Souza-Pinto, Nadja C; Wilson 3rd, David M; Stevnsner, Tinna V et al. (2008) Mitochondrial DNA, base excision repair and neurodegeneration. DNA Repair (Amst) 7:1098-109