Studies on an anti-trypanosomal factor (ATF) from the bacterial species Pseudomonas fluorescens disclosed that adsorption chromatography with high performance silica columns removed most of the inactive components from the ATF, yielding a fraction which was markedly purer. Counter current chromatography (Ito Coil) aided significantly in the production of larger amounts of the purer substance. Both techniques were useful in removing most of the lipopolysaccharide from the lytic fractions, providing a definite advantage for future in vivo chemotherapeutic studies against Trypanosoma cruzi.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000097-28
Application #
3960436
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
28
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code