This project has focused on the allergic inflammatory response seen in tissues after allergen exposure. In the first part of the project, the identification of the cells found in the human nasal mucosa were carefully measured, and then the effects of allergen exposure were examined. The resident cells consisted of lymphocytes, mast cells, and plasma cells. After allergen exposure, the major changes were activation of the lymphocyte (increased expression of the IL-2 receptor), an increase in eosinophil infiltration, and degranulation of mast cells. The bulk of the time on this project this year was spent developing techniques to measure cytokine expression quantitatively, to assess lymphocyte (increased expression of the IL-2 receptor), an increase in eosinophil infiltration, and degranulation of mast cells. The bulk of the time on this project this year was spent developing techniques to measure cytokine expression quantitatively, to assess lymphocyte phenotypes by FACS analysis, and to clone the human H-1 receptor.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000154-19
Application #
3768734
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
19
Fiscal Year
1993
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code