During the past year, to advance the goals of this project, crystals were generated from Ebola Zaire glycoprotein. Proteins with a trimerizing domain were produced,purified, and screened to determine which produced stable protein in a trimer conformation. One was identified, and crystallization methods were optimized to generate larger crystals to be analyzed at the national synchrotron x-ray research facility, APS, in Chicago, to determine whether they diffract. The crystals will be used to conduct mechanistic studies of the Ebola glycoprotein.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI005070-04
Application #
7592420
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2007
Total Cost
$295,946
Indirect Cost
City
State
Country
United States
Zip Code