Abstract

The Experimental Immunology Branch (EIB) flow cytometry laboratory currently supports multiple research projects for more than 50 investigators. These investigations involve multiparametric quantitative single cell analysis of, and electronic cell separation based upon, parameters associated with cells freshly prepared from different species and/or tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB and to other investigators within the Center for Cancer Research, NCI. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra-cellular signaling via cell surface molecules; b) analyses of cellular processes and/or defects in animals and/or cells with genetic modifications; c) studies of the mechanisms and consequences of immune pathogenesis; d) analyses of the coordinate cell surface expression of a variety of molecules; e) investigations of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in T cell lineage development; and i) mechanisms of cell death.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC009255-32
Application #
7592595
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
32
Fiscal Year
2007
Total Cost
$1,546,088
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Cohen, Helit; Parekh, Palak; Sercan, Zeynep et al. (2009) In vivo expression of MHC class I genes depends on the presence of a downstream barrier element. PLoS One 4:e6748
Chiang, Y Jeffrey; Jordan, Martha S; Horai, Reiko et al. (2009) Cbl enforces an SLP76-dependent signaling pathway for T cell differentiation. J Biol Chem 284:4429-38
Belkina, Natalya V; Liu, Yin; Hao, Jian-Jiang et al. (2009) LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation. Proc Natl Acad Sci U S A 106:4707-12
Leonard, Joshua N; Ghirlando, Rodolfo; Askins, Janine et al. (2008) The TLR3 signaling complex forms by cooperative receptor dimerization. Proc Natl Acad Sci U S A 105:258-63
Terabe, Masaki; Tagaya, Yutaka; Zhu, Qing et al. (2008) IL-15 expands unconventional CD8alphaalphaNK1.1+ T cells but not Valpha14Jalpha18+ NKT cells. J Immunol 180:7276-86
Walseng, Even; Bakke, Oddmund; Roche, Paul A (2008) Major histocompatibility complex class II-peptide complexes internalize using a clathrin- and dynamin-independent endocytosis pathway. J Biol Chem 283:14717-27
Puri, Niti; Roche, Paul A (2008) Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms. Proc Natl Acad Sci U S A 105:2580-5
Lutsiak, M E Christine; Tagaya, Yutaka; Adams, Anthony J et al. (2008) Tumor-induced impairment of TCR signaling results in compromised functionality of tumor-infiltrating regulatory T cells. J Immunol 180:5871-81
Williams, Joy A; Lumsden, Joanne M; Yu, Xiang et al. (2008) Regulation of thymic NKT cell development by the B7-CD28 costimulatory pathway. J Immunol 181:907-17
Chiang, Y Jeffrey; Hsiao, Susan J; Yver, Dena et al. (2008) Tankyrase 1 and tankyrase 2 are essential but redundant for mouse embryonic development. PLoS One 3:e2639

Showing the most recent 10 out of 56 publications