Abstract

We are interested in understanding the cell biology of T cell activation. We are examining the trafficking and localization of T cell ligands on antigen presenting cells both within these cells as well as on their plasma membranes. These studies are focused on understanding the molecular events leading to vesicular traffic, vesicle fusion with the plasma membrane, and the role of plasma membrane lipid microdomains in T cell activation. The orderly transport of proteins through the secretory pathway of eukaryotic cells is mediated by the recognition of donor membrane-derived vesicles with distinct target organelles. The specificity of this interaction is mediated in part by the specific interaction of membrane proteins termed SNAREs. Our laboratory is identifying novel SNAREs that regulate protein transport in an attempt to understand the molecular basis of protein traffic and exocytosis in lymphocytes

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Basic Sciences - NCI (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC009404-10
Application #
7048968
Study Section
(EIB)
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
2004
Total Cost
Indirect Cost

  Institution

Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Anderson, Howard A; Roche, Paul A (2015) MHC class II association with lipid rafts on the antigen presenting cell surface. Biochim Biophys Acta 1853:775-80
Berger, Adam C; Roche, Paul A (2009) MHC class II transport at a glance. J Cell Sci 122:1-4
Bryceson, Yenan T; Rudd, Eva; Zheng, Chengyun et al. (2007) Defective cytotoxic lymphocyte degranulation in syntaxin-11 deficient familial hemophagocytic lymphohistiocytosis 4 (FHL4) patients. Blood 110:1906-15
Muntasell, Aura; Berger, Adam C; Roche, Paul A (2007) T cell-induced secretion of MHC class II-peptide complexes on B cell exosomes. EMBO J 26:4263-72
Puri, Niti; Roche, Paul A (2006) Ternary SNARE complexes are enriched in lipid rafts during mast cell exocytosis. Traffic 7:1482-94
Roche, Paul A (2006) Gold-plating MHC class II molecules. Nat Chem Biol 2:178-9
Poloso, Neil J; Muntasell, Aura; Roche, Paul A (2004) MHC class II molecules traffic into lipid rafts during intracellular transport. J Immunol 173:4539-46
Poloso, Neil J; Roche, Paul A (2004) Association of MHC class II-peptide complexes with plasma membrane lipid microdomains. Curr Opin Immunol 16:103-7
Hiltbold, Elizabeth M; Poloso, Neil J; Roche, Paul A (2003) MHC class II-peptide complexes and APC lipid rafts accumulate at the immunological synapse. J Immunol 170:1329-38
Puri, Niti; Kruhlak, Michael J; Whiteheart, Sidney W et al. (2003) Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly. J Immunol 171:5345-52

Showing the most recent 10 out of 17 publications