Sequencing of the 45kb fragment of the HLA class III region from six individuals was completed by the Sanger Center over this past fiscal year, where mini-libraries were created from each PCR fragment and multiple clones were sequenced to create contigs. Further sequencing of short stretches that were not resolved by the Sanger Center are being completed in my laboratory. Meanwhile, Beena Neelam from Dr. Bob Stephens group has begun to assemble the contigs of genomic sequences from individual clone sequences provided by the Sanger Center and we are in the process of preliminary analysis of the data available so far to see if there is a correlation with the rate of recombination.KIR and HLA typing have been completed over this past year on the 30 geographically distinct populations, and in collaboration with Dr. Rich Single at the University of Vermont, we have begun to analyze the data.
| Gao, Xiaojiang; Single, Richard M; Karacki, Peter et al. (2006) Diversity of MICA and linkage disequilibrium with HLA-B in two North American populations. Hum Immunol 67:152-8 |
| Traherne, James A; Horton, Roger; Roberts, Anne N et al. (2006) Genetic analysis of completely sequenced disease-associated MHC haplotypes identifies shuffling of segments in recent human history. PLoS Genet 2:e9 |
| Malkki, M; Single, R; Carrington, M et al. (2005) MHC microsatellite diversity and linkage disequilibrium among common HLA-A, HLA-B, DRB1 haplotypes: implications for unrelated donor hematopoietic transplantation and disease association studies. Tissue Antigens 66:114-24 |
| Carrington, Mary; Cullen, Michael (2004) Justified chauvinism: advances in defining meiotic recombination through sperm typing. Trends Genet 20:196-205 |
| Gourraud, P A; Mano, S; Barnetche, T et al. (2004) Integration of microsatellite characteristics in the MHC region: a literature and sequence based analysis. Tissue Antigens 64:543-55 |
