Abstract

Gene Discovery ? Immunotherapy has been most successful when targeted to differentiation antigens expressed on hematopoietic tumors, in large part because the target antigens (e.g., CD20, CD25) are not expressed on essential cells or organs. Other cancers in which this approach could be successful are prostate, breast and ovary. To identify target antigens in prostate we have developed a computer based method to search the EST data base for ESTs that are expressed in prostate, prostate cancer and not other essential organs or cells. We have validated this approach experimentally and used it to identify prostate specific members of the MAGE/GAGE family which we term PAGE. We also found evidence for T-cell receptor gamma transcripts in prostate epithelial cells. This method promises to be a successful approach to the discovery of new targets in immunotherapy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC010298-01
Application #
6101084
Study Section
Special Emphasis Panel (LMB)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Sathyanarayana, Bangalore K; Hahn, Yoonsoo; Patankar, Manish S et al. (2009) Mesothelin, Stereocilin, and Otoancorin are predicted to have superhelical structures with ARM-type repeats. BMC Struct Biol 9:1
Ise, Tomoko; Das, Sudipto; Nagata, Satoshi et al. (2008) Expression of POTE protein in human testis detected by novel monoclonal antibodies. Biochem Biophys Res Commun 365:603-8
Levitin, Fiana; Weiss, Mordechai; Hahn, Yoonsoo et al. (2008) PATE gene clusters code for multiple, secreted TFP/Ly-6/uPAR proteins that are expressed in reproductive and neuron-rich tissues and possess neuromodulatory activity. J Biol Chem 283:16928-39
Epel, Malka; Carmi, Irit; Soueid-Baumgarten, Sharon et al. (2008) Targeting TARP, a novel breast and prostate tumor-associated antigen, with T cell receptor-like human recombinant antibodies. Eur J Immunol 38:1706-20
Bera, Tapan K; Saint Fleur, Ashley; Ha, Duc et al. (2008) Selective POTE paralogs on chromosome 2 are expressed in human embryonic stem cells. Stem Cells Dev 17:325-32
Das, Sudipto; Hahn, Yoonsoo; Walker, Dawn A et al. (2008) Topology of NGEP, a prostate-specific cell:cell junction protein widely expressed in many cancers of different grade level. Cancer Res 68:6306-12
Das, Sudipto; Ise, Tomoko; Nagata, Satoshi et al. (2007) Palmitoylation of POTE family proteins for plasma membrane targeting. Biochem Biophys Res Commun 363:751-6
Yokokawa, Junko; Bera, Tapan K; Palena, Claudia et al. (2007) Identification of cytotoxic T-lymphocyte epitope(s) and its agonist epitope(s) of a novel target for vaccine therapy (PAGE4). Int J Cancer 121:595-605
Das, Sudipto; Hahn, Yoonsoo; Nagata, Satoshi et al. (2007) NGEP, a prostate-specific plasma membrane protein that promotes the association of LNCaP cells. Cancer Res 67:1594-601
Korherr, Christian; Gille, Hendrik; Schafer, Rolf et al. (2006) Identification of proangiogenic genes and pathways by high-throughput functional genomics: TBK1 and the IRF3 pathway. Proc Natl Acad Sci U S A 103:4240-5

Showing the most recent 10 out of 24 publications