Abstract

The facility has two objectives. First, its mission is to provide state-of-the-art light microscopy instrumentation for the Lab of Receptor Biology and Gene Expression. Second, the facility provides support to anyone in the CCR who requires assistance with fluorescence imaging. Present work in the facility revolves around four different general techniques: structural studies such as colocalization of different fluorescent markers, time-lapse analysis to follow how cellular components change over time, fluorescence recovery after photobleaching (FRAP)to measure mobilities and binding of cell molecues and fluorescence resonance energy transfer (FRET) to measure molecular interactions in live or fixed specimens.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC010571-04
Application #
7592770
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2007
Total Cost
$247,119
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Koga, Fumitaka; Xu, Wanping; Karpova, Tatiana S et al. (2006) Hsp90 inhibition transiently activates Src kinase and promotes Src-dependent Akt and Erk activation. Proc Natl Acad Sci U S A 103:11318-22
Arnaoutov, Alexei; Azuma, Yoshiaki; Ribbeck, Katharina et al. (2005) Crm1 is a mitotic effector of Ran-GTP in somatic cells. Nat Cell Biol 7:626-32
Laricchia-Robbio, Leopoldo; Tamura, Tomohiko; Karpova, Tatiana et al. (2005) Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages. Proc Natl Acad Sci U S A 102:14368-73
Catalfamo, Marta; Karpova, Tatiana; McNally, James et al. (2004) Human CD8+ T cells store RANTES in a unique secretory compartment and release it rapidly after TcR stimulation. Immunity 20:219-30
Kim, Byung-Chul; Lee, Ho-Jae; Park, Seok Hee et al. (2004) Jab1/CSN5, a component of the COP9 signalosome, regulates transforming growth factor beta signaling by binding to Smad7 and promoting its degradation. Mol Cell Biol 24:2251-62
Qian, Xiaolan; Karpova, Tatiana; Sheppard, Allan M et al. (2004) E-cadherin-mediated adhesion inhibits ligand-dependent activation of diverse receptor tyrosine kinases. EMBO J 23:1739-48
Ling, Yan; Sankpal, Umesh T; Robertson, Andrea K et al. (2004) Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription. Nucleic Acids Res 32:598-610
Wong, Kasuen; Zhang, Junyu; Awasthi, Soumya et al. (2004) Nerve growth factor receptor signaling induces histone acetyltransferase domain-dependent nuclear translocation of p300/CREB-binding protein-associated factor and hGCN5 acetyltransferases. J Biol Chem 279:55667-74
Park, Jung-Eun; Park, Chong J; Sakchaisri, Krisada et al. (2004) Novel functional dissection of the localization-specific roles of budding yeast polo kinase Cdc5p. Mol Cell Biol 24:9873-86
Edghill-Smith, Yvette; Venzon, David; Karpova, Tatiana et al. (2003) Modeling a safer smallpox vaccination regimen, for human immunodeficiency virus type 1-infected patients, in immunocompromised macaques. J Infect Dis 188:1181-91

Showing the most recent 10 out of 16 publications