Our lab has focused on apoB mRNA editing as a model of post- transcriptional base modification in humans. ApoB mRNA editing is a site specific deamination of a cytidine residue to a uridine at nucleotide position 6666 in the ApoB mRNA transcript. The deaminination reaction is a zinc dependent process mediated by a cytadine deaminase referred to as apolipoprotein B editing component 1 (APOBEC-1), RNA editing activity of APOBEC-1 is trictly dependent on assembly on an enzyme complex that includes APOBEC-1(the catalytic subunit), and auxiliary proteins. During the past year, we have examined regulation of apoB mRNA editing by analysis of post-transcriptional proetin phosphorylation.
