A project has been initiated to produce by genetic engineering, a single polypeptide chain with the antigen binding ability of a bispecific antibody. We have produced several cloned plasmids containing inserts encoding single chain Fv (sFv) molecules. One of these, an anti-DNP sFv, has been expressed in E. coli as inclusion bodies. This protein has been refolded into a molecule with antigen-binding activity.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CB009289-04
Application #
3796556
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Division of Cancer Biology and Diagnosis
Department
Type
DUNS #
City
State
Country
United States
Zip Code