Monoclonal antibodies were screened for the ability to block the motility of human A2058 melanoma cells. A monoclonal antibody was identified which arrested tumor cell motility without altering adhesion. The antigen recognized by the antibody is a glycosylated cell surface protein with a molecular weight of 95 kDa. This antibody was used to screen a cDNA library. The cDNA clone isolated encodes a unique protein. Homology is noted with CD4, N-CAM and certain ion channel proteins. The predicted sequence has a transmembrane domain. Future plans include a structure-function analysis of this protein as a regulatory of motility and a correlative analysis of the levels of the protein or its mRNA associated with various stages of tumor progression.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CB009352-01
Application #
3808617
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Division of Cancer Biology and Diagnosis
Department
Type
DUNS #
City
State
Country
United States
Zip Code