A gene has been isolated from an activated T cell library that appears to encode a novel low molecular weight GTP-binding protein that demonstrates approximately 20 percent homology to c-Ha-ras. This gene has been designated RAI-1 for transcriptionally induced ras-related protein. RAI-1 codes for a 35 kdalton phosphoprotein that contains consensus GTP binding elements, although the G-3 element is imperfect. RAI-1 has been shown to preferentially bind GTP over ATP. RAI-1 differs from c-ras proteins by the presence of amino and carboxy-terminal extensions and the absence of an acceptor site for isoprenylation. RAI-1 message is transiently expressed in mitogen-activated cells of various lineages. RAI-1 appears to be associated with the endoplasmic reticulum, most likely through an association at the cytoplasmic face. The GTP-binding property of RAI-1 in addition to its phosphorylation (which can often be regulatory for function) suggest the possibility that RAI-1 is a signal transducing protein associated with communication between the lumen of the ER and the cytoplasm.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CB009357-02
Application #
3796567
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Division of Cancer Biology and Diagnosis
Department
Type
DUNS #
City
State
Country
United States
Zip Code