Abstract

The collection of human blood from both patients and healthy volunteers is necessary for the development of laboratory assays required for studies of the role of nitric oxide, inflammatory mediators and endothelial function in inflammatory diseases that involve the blood vessels. To date we have enrolled 163 individuals and collected blood numerous times to be used in experimental assays. Some of these samples have been used for gene expression studies and nitrite bioavailability studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL008050-04
Application #
7332155
Study Section
(CCMD)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2006
Total Cost
Indirect Cost

  Institution

Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Nahavandi, M; Nichols, J P; Hassan, M et al. (2009) Near-infrared spectra absorbance of blood from sickle cell patients and normal individuals. Hematology 14:46-8
Wang, Xunde; Kettenhofen, Nicholas J; Shiva, Sruti et al. (2008) Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins. Free Radic Biol Med 44:1362-72
Raghavachari, Nalini; Xu, Xiuli; Harris, Amy et al. (2007) Amplified expression profiling of platelet transcriptome reveals changes in arginine metabolic pathways in patients with sickle cell disease. Circulation 115:1551-62
Basu, Swati; Grubina, Rozalina; Huang, Jinming et al. (2007) Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin. Nat Chem Biol 3:785-94
Villagra, Jose; Shiva, Sruti; Hunter, Lori A et al. (2007) Platelet activation in patients with sickle disease, hemolysis-associated pulmonary hypertension, and nitric oxide scavenging by cell-free hemoglobin. Blood 110:2166-72
Wang, Xunde; Bryan, Nathan S; MacArthur, Peter H et al. (2006) Measurement of nitric oxide levels in the red cell: validation of tri-iodide-based chemiluminescence with acid-sulfanilamide pretreatment. J Biol Chem 281:26994-7002
Pelletier, Mildred M; Kleinbongard, Petra; Ringwood, Lorna et al. (2006) The measurement of blood and plasma nitrite by chemiluminescence: pitfalls and solutions. Free Radic Biol Med 41:541-8
Shiva, Sruti; Wang, Xunde; Ringwood, Lorna A et al. (2006) Ceruloplasmin is a NO oxidase and nitrite synthase that determines endocrine NO homeostasis. Nat Chem Biol 2:486-93
Huang, Zhi; Shiva, Sruti; Kim-Shapiro, Daniel B et al. (2005) Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control. J Clin Invest 115:2099-107
Piknova, Barbora; Gladwin, Mark T; Schechter, Alan N et al. (2005) Electron paramagnetic resonance analysis of nitrosylhemoglobin in humans during NO inhalation. J Biol Chem 280:40583-8

Showing the most recent 10 out of 12 publications