The Jaffe reaction (creatinine and alkaline picrate) was studied to elucidate its spectral mechanism and to determine which functional groups are necessary or sufficient for the reaction to occur. 1. Interferences: Only by knowing the molecular mechanism can predictions be made from structure as to probability of interference. We established that the reaction does not occur through the formation of a carbanion adjacent to the ketone. 2. Suitable evidence was obtained to infer that the carbonyl or a nitrogen group is necessary for reactivity and to suggest that the Jaffe reaction involves a charge transfer complex.

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL010085-07
Application #
3916514
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code