There are many factors that influence the addictiveness of a drug. The route of administration is a major determinant of the speed of drug entry into the body and to receptors in the central nervous system which produce resultant effects. Clinical studies are in progress which determine the importance of different routes of administration and the relationship of blood concentrations of drug and active metabolites to concomitant drug effects. Studies of heroin, cocaine, methamphetamine, codeine and marijuana administration by the intravenous, smoked, intranasal and oral routes are underway or have been performed. In order to study the pharmacokinetics of chronic cocaine use, multiple oral doses of cocaine are administered and concurrent physiological, behavioral and performance measures are collected along with blood specimens. Another new study examines oral administration of tetrahydrocannabinol, the primary psychoactive component of marijuana, in the form of Dronabinol (synthetic tetrahydrocannabinol) and hemp oil products. Pharmacodynamic effects include subjective, cognitive and psychomotor performance and physiological measures. Plasma, urine, oral fluid, sweat, hair, nails and skin are analyzed for drug and metabolites by solid phase extraction-gas chromatography/mass spectrometry. New analytical approaches, e.g., liquid chromatography/mass spectrometry, liquid chromatography/MS/MS and chemical ionization gas chromatography/mass spectrometry, have been added to study the disposition of drug and metabolites in these alternative biological matrices. The intravenous and smoked routes produce a rapid onset of pharmacological effects together with an early appearance of drug and metabolites in blood. Evidence of delays in distribution to effector sites are observed following marijuana smoking, but drug effects following heroin and cocaine administration coincided more closely with concurrent blood concentrations. Onset of effects is delayed longer following intranasal administration and became pronounced after oral administration.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Intramural Research (Z01)
Project #
1Z01DA000414-06
Application #
6830632
Study Section
(CPTB)
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2003
Total Cost
Indirect Cost
Name
National Institute on Drug Abuse
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Muniyappa, Ranganath; Sable, Sara; Ouwerkerk, Ronald et al. (2013) Metabolic Effects of Chronic Cannabis Smoking. Diabetes Care :
Lee, Dayong; Karschner, Erin L; Milman, Garry et al. (2013) Can oral fluid cannabinoid testing monitor medication compliance and/or cannabis smoking during oral THC and oromucosal Sativex administration? Drug Alcohol Depend 130:68-76
Desrosiers, Nathalie A; Barnes, Allan J; Hartman, Rebecca L et al. (2013) Oral fluid and plasma 3,4-methylenedioxymethamphetamine (MDMA) and metabolite correlation after controlled oral MDMA administration. Anal Bioanal Chem 405:4067-76
Concheiro, Marta; Jones, HendreƩ; Johnson, Rolley E et al. (2010) Confirmatory analysis of buprenorphine, norbuprenorphine, and glucuronide metabolites in plasma by LCMSMS. Application to umbilical cord plasma from buprenorphine-maintained pregnant women. J Chromatogr B Analyt Technol Biomed Life Sci 878:13-20
Milman, Garry; Barnes, Allan J; Lowe, Ross H et al. (2010) Simultaneous quantification of cannabinoids and metabolites in oral fluid by two-dimensional gas chromatography mass spectrometry. J Chromatogr A 1217:1513-21
Barnes, Allan J; Smith, Michael L; Kacinko, Sherri L et al. (2008) Excretion of methamphetamine and amphetamine in human sweat following controlled oral methamphetamine administration. Clin Chem 54:172-80
Kacinko, Sherri L; Shakleya, Diaa M; Huestis, Marilyn A (2008) Validation and application of a method for the determination of buprenorphine, norbuprenorphine, and their glucuronide conjugates in human meconium. Anal Chem 80:246-52
Huestis, Marilyn A; Scheidweiler, Karl B; Saito, Takeshi et al. (2008) Excretion of Delta9-tetrahydrocannabinol in sweat. Forensic Sci Int 174:173-7
Cone, Edward J; Huestis, Marilyn A (2007) Interpretation of oral fluid tests for drugs of abuse. Ann N Y Acad Sci 1098:51-103
Huestis, Marilyn A (2007) Human cannabinoid pharmacokinetics. Chem Biodivers 4:1770-804

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