Abstract

We have studied the distribution of the histone variants H3.3 and H2A.Z in chicken erythroid cells. We had shown recently that H3.3 is concentrated at many regulatory regions, as well as over the transcribed regions of some active genes. We have now shown that nucleosomes containing H3.3 are less stable than those containing the predominant species, H3.1, and lose their H2AH2B components more readily. Furthermore, nucleosomes containing H3.3 and H2A.Z are even more unstable. Using double chromatin immunoprecipitation methods we have identified numerous genomic loci at where such doubly substituted nucleosomes are present. They mark transcriptional regulatory sites that control active genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Intramural Research (Z01)
Project #
1Z01DK036149-01
Application #
7593566
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2007
Total Cost
$364,594
Indirect Cost

  Institution

Name
National Institute of Diabetes and Digestive and Kidney Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Iwata, Yasunori; Boström, Elisabeth A; Menke, Julia et al. (2012) Aberrant macrophages mediate defective kidney repair that triggers nephritis in lupus-susceptible mice. J Immunol 188:4568-80
Menke, Julia; Kriegsmann, Jörg; Schimanski, Carl Christoph et al. (2012) Autocrine CSF-1 and CSF-1 receptor coexpression promotes renal cell carcinoma growth. Cancer Res 72:187-200
Menke, Julia; Bork, Tillmann; Kutska, Birte et al. (2011) Targeting transcription factor Stat4 uncovers a role for interleukin-18 in the pathogenesis of severe lupus nephritis in mice. Kidney Int 79:452-63
Menke, Julia; Iwata, Yasunori; Rabacal, Whitney A et al. (2011) Distinct roles of CSF-1 isoforms in lupus nephritis. J Am Soc Nephrol 22:1821-33
Giles, K E; Gowher, H; Ghirlando, R et al. (2010) Chromatin boundaries, insulators, and long-range interactions in the nucleus. Cold Spring Harb Symp Quant Biol 75:79-85
Menke, Julia; Rabacal, Whitney A; Byrne, Katelyn T et al. (2009) Circulating CSF-1 promotes monocyte and macrophage phenotypes that enhance lupus nephritis. J Am Soc Nephrol 20:2581-92
Menke, Julia; Iwata, Yasunori; Rabacal, Whitney A et al. (2009) CSF-1 signals directly to renal tubular epithelial cells to mediate repair in mice. J Clin Invest 119:2330-42
Jin, Chunyuan; Felsenfeld, Gary (2007) Nucleosome stability mediated by histone variants H3.3 and H2A.Z. Genes Dev 21:1519-29
Jin, Chunyuan; Felsenfeld, Gary (2006) Distribution of histone H3.3 in hematopoietic cell lineages. Proc Natl Acad Sci U S A 103:574-9