Fast atom bombardment (FAB) has become one of the most widely utilized techniques for obtaining mass spectra of thermally labile and/or very polar substances. In conjunction with tandem MS (MS- MS), the fragmentation information so vital for structure elucidation can be obtained from ions normally not observed to fragment and from mixtures where the parent ion-daughter ion relationships are not known. This project involves instrumental developments in MS-MS and application of FAB/MS and FAB/MS-MS for structure elucidation. In the area of instrumental developments we are currently investigating the effect of energy loss on the MS-MS data obtained by a linked scan of MS-II and for how these effects can best be compensated. Applications of FAB/MS and FAB/MS/MS for structure elucidation include: the structure elucidation of phospholipids containing unusual fatty acid and headgroups; peptides in which an amide linkage has been modified; and glutathione, cysteine and N- acetylcysteine conjugates of xenobiotics, both synthetic and conjugates isolated from challenged animals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES050098-04
Application #
3898113
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1989
Total Cost
Indirect Cost
Name
U.S. National Inst of Environ Hlth Scis
Department
Type
DUNS #
City
State
Country
United States
Zip Code