Abstract

For prevention and cure of AIDS, it is important to know the mechanism of how cell lineage for T lymphocyte is determined during embryonic development. T lymphocytes are derived from hematopoietic stem cells, however, it is still unclear how hematopoietic cells are developed from undifferentiated mesodermal tissues. We are focusing on the function of Bone Morphogenetic Proteins (BMPs), the members of TGF-beta superfamily, during mouse development. BMPs have function to specify the fate of mesodermal tissues more ventral. Hematopoietic cells are derived from the most ventral mesoderm, therefore, BMPs are hypothesized to play important roles during specification of T lymphocyte lineage in the hematopietic tissues. To reveal the function of BMP signaling during hematopoiesis, we will set up hematopoietic cell specific disruption of BMP receptor genes using embryonic stem cell technology. The knowledge that will be acquired through this research may give us better understanding for etilogy of AIDS and hopefully for prevention and cure of this fatal disease. - AIDS, ES cells embryo culture tissue specific knock-out

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES071004-01
Application #
6227947
Study Section
Special Emphasis Panel (LRDT)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
National Institute of Environmental Health Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Miura, Shigeto; Mishina, Yuji (2007) The DVE changes distal epiblast fate from definitive endoderm to neurectoderm by antagonizing nodal signaling. Dev Dyn 236:1602-10
Di-Gregorio, Aida; Sancho, Margarida; Stuckey, Daniel W et al. (2007) BMP signalling inhibits premature neural differentiation in the mouse embryo. Development 134:3359-69
Song, Lanying; Fassler, Reinhard; Mishina, Yuji et al. (2007) Essential functions of Alk3 during AV cushion morphogenesis in mouse embryonic hearts. Dev Biol 301:276-86
Miura, Shigeto; Davis, Shannon; Klingensmith, John et al. (2006) BMP signaling in the epiblast is required for proper recruitment of the prospective paraxial mesoderm and development of the somites. Development 133:3767-75
Park, Changwon; Lavine, Kory; Mishina, Yuji et al. (2006) Bone morphogenetic protein receptor 1A signaling is dispensable for hematopoietic development but essential for vessel and atrioventricular endocardial cushion formation. Development 133:3473-84
Kishigami, Satoshi; Komatsu, Yoshihiro; Takeda, Haruko et al. (2006) Optimized beta-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos. Genesis 44:57-65
Fukuda, Tomokazu; Scott, Gregory; Komatsu, Yoshihiro et al. (2006) Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2. Genesis 44:159-67
Kishigami, Satoshi; Mishina, Yuji (2005) BMP signaling and early embryonic patterning. Cytokine Growth Factor Rev 16:265-78
Gaussin, Vinciane; Morley, Gregory E; Cox, Luk et al. (2005) Alk3/Bmpr1a receptor is required for development of the atrioventricular canal into valves and annulus fibrosus. Circ Res 97:219-26
Oh, Hidemasa; Chi, Xuan; Bradfute, Steven B et al. (2004) Cardiac muscle plasticity in adult and embryo by heart-derived progenitor cells. Ann N Y Acad Sci 1015:182-9

Showing the most recent 10 out of 16 publications