All non-sensory pertussis sensitive G proteins have been inactivated in mice in prior years by gene targeting. Gi2-/- mice develop ulcerative colitis, Go-/- mice develop a turning syndrome. During this year we concentrated on breeding the Gi2 mutation into mice a proper background to allow us to map susceptibility genes responsible for lack of ulcerative colitis in C57BL/6J mice but make it 100% penetrant in 129Sv mice. In collaborative studies we found that Go alpha counteracts the positive effects of cAMP in neurite outgrowth and on N-type Ca channel synthesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES101643-01
Application #
6828645
Study Section
(LST)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2003
Total Cost
Indirect Cost
Name
U.S. National Inst of Environ Hlth Scis
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Marshall, John; Zhou, Xiao-Zhong; Chen, Gang et al. (2018) Antidepression action of BDNF requires and is mimicked by G?i1/3 expression in the hippocampus. Proc Natl Acad Sci U S A 115:E3549-E3558
Bissinger, Rosi; Lang, Elisabeth; Ghashghaeinia, Mehrdad et al. (2016) Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit G?i2. Sci Rep 6:30925
Choi, Jung-Mi; Kim, Sung-Soo; Choi, Chan-Il et al. (2016) Development of the main olfactory system and main olfactory epithelium-dependent male mating behavior are altered in Go-deficient mice. Proc Natl Acad Sci U S A 113:10974-9
Xu, Ying; Orlandi, Cesare; Cao, Yan et al. (2016) The TRPM1 channel in ON-bipolar cells is gated by both the ? and the ?? subunits of the G-protein Go. Sci Rep 6:20940
Li, Xianjing; Wang, Duowei; Chen, Zhen et al. (2015) G?i1 and G?i3 regulate macrophage polarization by forming a complex containing CD14 and Gab1. Proc Natl Acad Sci U S A 112:4731-6
Birnbaumer, Lutz (2015) From GTP and G proteins to TRPC channels: a personal account. J Mol Med (Berl) 93:941-53
Devanathan, Vasudharani; Hagedorn, Ina; Köhler, David et al. (2015) Platelet Gi protein G?i2 is an essential mediator of thrombo-inflammatory organ damage in mice. Proc Natl Acad Sci U S A 112:6491-6
Pérez-Gómez, Anabel; Bleymehl, Katherin; Stein, Benjamin et al. (2015) Innate Predator Odor Aversion Driven by Parallel Olfactory Subsystems that Converge in the Ventromedial Hypothalamus. Curr Biol 25:1340-6
Keller, Kirsten; Maass, Martina; Dizayee, Sara et al. (2015) Lack of G?i2 leads to dilative cardiomyopathy and increased mortality in ?1-adrenoceptor overexpressing mice. Cardiovasc Res 108:348-56
Villanueva, Hugo; Visbal, Adriana P; Obeid, Nadine F et al. (2015) An essential role for G?(i2) in Smoothened-stimulated epithelial cell proliferation in the mammary gland. Sci Signal 8:ra92

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