The aim of this project is to examine the involvement of certain ribonucleases in cellular events. The scope of this project has evolved to include protein chemistry, enzymatic activity, overproduction of proteins and the consequences of changes in the level of enzymatic activity within the cell. The basis for most of the experimental approaches is derived from an enzymological perspective. The ability to examine a particular event in great detail from primary amino acid sequence of a protein to the consequences of alteration of the level of enzymatic activity within the cell has given us the opportunity to study both the enzymological and functional roles of these intriguing enzymes. A second area of interest is mutagenesis in E. coli.
| Nowotny, Marcin; Gaidamakov, Sergei A; Ghirlando, Rodolfo et al. (2007) Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. Mol Cell 28:264-76 |
| Budihas, Scott R; Gorshkova, Inna; Gaidamakov, Sergei et al. (2005) Selective inhibition of HIV-1 reverse transcriptase-associated ribonuclease H activity by hydroxylated tropolones. Nucleic Acids Res 33:1249-56 |
| Gaidamakov, Sergei A; Gorshkova, Inna I; Schuck, Peter et al. (2005) Eukaryotic RNases H1 act processively by interactions through the duplex RNA-binding domain. Nucleic Acids Res 33:2166-75 |
| Nowotny, Marcin; Gaidamakov, Sergei A; Crouch, Robert J et al. (2005) Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell 121:1005-16 |
| Chan, King C; Budihas, Scott R; Le Grice, Stuart F J et al. (2004) A capillary electrophoretic assay for ribonuclease H activity. Anal Biochem 331:296-302 |
| Jeong, Ho-Sang; Backlund, Peter S; Chen, Hao-Chia et al. (2004) RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. Nucleic Acids Res 32:407-14 |
| Broccoli, Sonia; Rallu, Fabien; Sanscartier, Patrick et al. (2004) Effects of RNA polymerase modifications on transcription-induced negative supercoiling and associated R-loop formation. Mol Microbiol 52:1769-79 |
| Backer, Marina V; Gaynutdinov, Timur I; Gorshkova, Inna I et al. (2003) Humanized docking system for assembly of targeting drug delivery complexes. J Control Release 89:499-511 |
| Pileur, Frederic; Andreola, Marie-Line; Dausse, Eric et al. (2003) Selective inhibitory DNA aptamers of the human RNase H1. Nucleic Acids Res 31:5776-88 |
| Cerritelli, Susana M; Frolova, Ella G; Feng, Chiguang et al. (2003) Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice. Mol Cell 11:807-15 |
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