Characterization of the 5'Regulatory Region of the Mouse (M) CGTP1 Gene

Napolitano, M.

Abstract

Seven cyclic nucleotide phosphodiesterase (PDE) gene families (Types I- VII) have been identified. We have cloned and expressed representatives of two sub-types of Type III cGMP-inhibited (cGI) PDEs, rat (R) and Human (H) cGIP1 and 2. A membrane-associated adipocyte cGI PDE (McGIP1 isoform) appears during differentiation of 3T3-L1 adipocytes. To learn more about the regulation of McGIP1 gene expression we cloned the cDNA for the cGI-PDE from a mouse adipocyte lambda gt 11 cDNA library (McGIP1 cDNA) The open reading frame of 3303 nucleotides is very similar to the sequence of RcGIP1 cDNA; the carboxyl-terminal region includes the putative catalytic domain highly conserved among mammalian PDEs. On Northern blots, McGIP1 cDNA hybridizes with a prominent approx. 5.5-kb band in poly(A)+ RNA from mouse adipose tissue, testis, liver, lung, and cerebellum, but not heart. Active recombinant McGIP1 was expressed in Sf9 cells transfected with recombinant baculovirus vector.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Intramural Research (Z01)
• Project #: 1Z01HL000654-05
• Application #: 5203496
• Support Year: 5
• Fiscal Year: 1995

Institution

• Name: National Heart, Lung, and Blood Institute
• Country: United States

Publications

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Coudray, C; Charon, C; Komas, N et al. (1999) Evidence for the presence of several phosphodiesterase isoforms in brown adipose tissue of Zucker rats: modulation of PDE2 by the fa gene expression. FEBS Lett 456:207-10

Castan, I; Wijkander, J; Manganiello, V et al. (1999) Mechanisms of inhibition of lipolysis by insulin, vanadate and peroxovanadate in rat adipocytes. Biochem J 339 ( Pt 2):281-9