Studies of the reaction of NAD-NADASE have been extended on the all tantalum stopped-flow microcalorimeter to 10 degrees C, 25 degrees C, and 37 degrees C confirming the endothermic nature of the reaction by demonstrating the 5-fold increase in activity from 25 degrees C to 10 degrees C with the almost complete loss in activity at 37 degrees C. A new heat capacity measuring microcalorimeter has been developed which is capable of measuring charges of 1 part in one hundred thousand of total heat capacity of phospholipids. A reaction heat of 60 calories/mole was found as they went from multilamellar vesicles to unilamellar vesicles. The total change in heat capacity was 2 to 3% depending on the system.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL001414-18
Application #
3878934
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
1990
Total Cost
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code