Given that there are about 10- to 100-thousand genes and most mutations result in subtle phenotypes, a mutational analysis of all genes individually would be highly inefficient. Therefore, we have developed a method for systematically scanning the genome for interesting phenotypes by introducing deletions spanning up to a Megabase of chromosomal DNA. This """"""""deletion scanning"""""""" employs the Cre/lox system of site-specific recombination in murine embryonic stem cells and allows to systematically scan along a chromosome for mutations with interesting phenotypes. We have worked out and built the vector cassettes to receive the different genomic fragments spaced along a chromosome. We have worked out the conditions for co-transfection of Cre-recombinase with these vectors. We are now starting to proceed along mouse chromosome 16 with a systematic in vivo mutational analysis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Intramural Research (Z01)
Project #
1Z01MH002710-03
Application #
6162934
Study Section
Special Emphasis Panel (NSB)
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost
Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
United States
Zip Code