Abstract

We have continued to study the differentiation of bone marrow derived stem cells into tissue-specific differentiated cells. We examined postmortem brains of women who received bone marrow from male relatives. In several brain regions we colocalized neuronal markers (NeuN- a neuron specific nuclear marker and Kv2.1 - a neuron specific potassium channel marker) with the human Y chromosome. In all four patients studied we detected neurons derived from the donor bone marrow. Colocalizations were confirmed using confocal microscopy. The highest number of donor derived neurons was observed in the youngest patient, a 2 year old who survived longer than the other bone marrow recipients. The finding extends our earlier findings in rodents to humans.At present we are performing similar studies in samples from patiennts with longer survival times.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Intramural Research (Z01)
Project #
1Z01NS002996-02
Application #
6843285
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2003
Total Cost
Indirect Cost

  Institution

Name
National Institute of Neurological Disorders and Stroke
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Mezey, Eva (2004) Commentary: on bone marrow stem cells and openmindedness. Stem Cells Dev 13:147-52
Tran, Simon D; Pillemer, Stanley R; Dutra, Amalia et al. (2003) Differentiation of human bone marrow-derived cells into buccal epithelial cells in vivo: a molecular analytical study. Lancet 361:1084-8
Mezey, Eva; Key, Sharon; Vogelsang, Georgia et al. (2003) Transplanted bone marrow generates new neurons in human brains. Proc Natl Acad Sci U S A 100:1364-9
Mezey, Eva; Nagy, Andras; Szalayova, Ildiko et al. (2003) Comment on ""Failure of bone marrow cells to transdifferentiate into neural cells in vivo"". Science 299:1184; author reply 1184
Mallet, Vincent O; Mitchell, Claudia; Mezey, Eva et al. (2002) Bone marrow transplantation in mice leads to a minor population of hepatocytes that can be selectively amplified in vivo. Hepatology 35:799-804
Mezey, E; Chandross, K J (2000) Bone marrow: a possible alternative source of cells in the adult nervous system. Eur J Pharmacol 405:297-302
Mezey, E; Chandross, K J; Harta, G et al. (2000) Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow. Science 290:1779-82